Clostridium sordellii Phospholipase C: Gene Cloning and Comparison of Enzymatic and Biological Activities with Those of Clostridium perfringens and Clostridium bifermentans Phospholipase C

The gene encoding Clostridium sordellii phospholipase C (Csp) was cloned and expressed as a histidine-tagged (His-tag) protein, and the protein was purified to compare its enzymatic and biological activities with those of Clostridium perfringens phospholipase C (Cpa) and Clostridium bifermentans pho...

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Bibliographic Details
Main Authors: Karasawa, Tadahiro, Wang, Xingmin, Maegawa, Tsuneo, Michiwa, Yoshio, Kita, Hiroyuki, Miwa, Koichi, Nakamura, Shinichi
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2003
Subjects:
Molecular Pathogenesis
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC145374/
https://ncbi.nlm.nih.gov/pubmed/12540540
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.71.2.641-646.2003
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