"Per cell" normalization method for mRNA measurement by quantitative PCR and microarrays

BACKGROUND: Transcriptome data from quantitative PCR (Q-PCR) and DNA microarrays are typically obtained from a fixed amount of RNA collected per sample. Therefore, variations in tissue cellularity and RNA yield across samples in an experimental series compromise accurate determination of the absolut...

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Detalhes bibliográficos
Main Authors: Kanno, Jun, Aisaki, Ken-ichi, Igarashi, Katsuhide, Nakatsu, Noriyuki, Ono, Atsushi, Kodama, Yukio, Nagao, Taku
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2006
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1448209/
https://ncbi.nlm.nih.gov/pubmed/16571132
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-7-64
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