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Green Fluorescent Chimeras Indicate Nonpolar Localization of Pullulanase Secreton Components PulL and PulM

The Klebsiella oxytoca pullulanase secreton (type II secretion system) components PulM and PulL were tagged at their N termini with green fluorescent protein (GFP), and their subcellular location was examined by fluorescence microscopy and fractionation. When produced at moderate levels without othe...

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Detalhes bibliográficos
Main Authors: Buddelmeijer, Nienke, Francetic, Olivera, Pugsley, Anthony P.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1447010/
https://ncbi.nlm.nih.gov/pubmed/16585754
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.188.8.2928-2935.2006
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