A method for the separation of lymphocytes and plasma cells from the human palatine tonsil using sedimentation in an isokinetic gradient of Ficoll in tissue culture medium.

Several methods for the dissociation of human tonsils into cell suspensions were compared. Dissociation of tonsils using 0-25 per cent trypsin gave both the largest number of total cells and the largest number of plasma cells per gram of tonsil. Lymphocytes and plasma cells were separated in a previ...

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Detalles Bibliográficos
Main Authors: Willson, J K, Luberoff, D E, Pitts, A, Pretlow, T C
Formato: Artigo
Idioma:Inglês
Publicado: 1975
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1445752/
https://ncbi.nlm.nih.gov/pubmed/1090518
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