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Expression of Baculovirus Late and Very Late Genes Depends on LEF-4, a Component of the Viral RNA Polymerase Whose Guanyltransferase Function Is Essential

Baculovirus lef-4 encodes one subunit of the viral RNA polymerase. Here, we demonstrate the essential nature of LEF-4 by RNA interference and bacmid knockout technology. Silencing of LEF-4 in wild-type virus-infected cells suppressed expression of structural genes, while early expression was unaffec...

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Main Authors: Knebel-Mörsdorf, Dagmar, Quadt, Ilja, Li, Yi, Montier, Laura, Guarino, Linda A.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2006
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1440449/
https://ncbi.nlm.nih.gov/pubmed/16571832
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.80.8.4168-4173.2006
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