Cloning, Sequencing, and Expression of the Chitinase Gene chiA74 from Bacillus thuringiensis

The endochitinase gene chiA74 from Bacillus thuringiensis serovar kenyae strain LBIT-82 was cloned in Escherichia coli DH5αF′. A sequence of 676 amino acids was deduced when the gene was completely sequenced. A molecular mass of 74 kDa was estimated for the preprotein, which includes a putative 4-kD...

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Bibliografiske detaljer
Main Authors: Barboza-Corona, J. Eleazar, Nieto-Mazzocco, Elizabeth, Velázquez-Robledo, Rocio, Salcedo-Hernandez, Rubén, Bautista, Mayela, Jiménez, Beatriz, Ibarra, Jorge E.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2003
Fag:
Invertebrate Microbiology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC143672/
https://ncbi.nlm.nih.gov/pubmed/12571025
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.2.1023-1029.2003
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