Development of Sinorhizobium meliloti Pilot Macroarrays for Transcriptome Analysis

In order to prepare for whole-genome expression analysis in Sinorhizobium meliloti, pilot DNA macroarrays were designed for 34 genes of known regulation. The experimental parameters assessed were the length of the PCR products, the influence of a tag at the 5′ end of the primers, and the method of R...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Bergès, Hélène, Lauber, Emmanuelle, Liebe, Carine, Batut, Jacques, Kahn, Daniel, de Bruijn, Frans J., Ampe, Frédéric
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2003
Schlagworte:	Methods
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC143623/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12571049/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.2.1214-1219.2003
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