The bifunctional dihydrofolate reductase thymidylate synthase of Tetrahymena thermophila provides a tool for molecular and biotechnology applications

BACKGROUND: Dihydrofolate reductase (DHFR) and thymidylate synthase (TS) are crucial enzymes in DNA synthesis. In alveolata both enzymes are expressed as one bifunctional enzyme. RESULTS: Loss of this essential enzyme activities after successful allelic assortment of knock out alleles yields an auxo...

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Bibliografiske detaljer
Main Authors: Herrmann, Lutz, Bockau, Ulrike, Tiedtke, Arno, Hartmann, Marcus WW, Weide, Thomas
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2006
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1435751/
https://ncbi.nlm.nih.gov/pubmed/16549005
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-6-21
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