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ANS Fluorescence Detects Widespread Perturbations of Protein Tertiary Structure in Ice

Freeze-induced perturbations of the protein native fold are poorly understood owing to the difficulty of monitoring their structure in ice. Here, we report that binding of the fluorescence probe 1-anilino-8-naphthalene sulfonate (ANS) to proteins in ice can provide a general monitor of ice-induced a...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Gabellieri, Edi, Strambini, Giovanni B.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Biophysical Society 2006
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC1432122/
https://ncbi.nlm.nih.gov/pubmed/16461396
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.074948
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