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Biochemical and Mutational Characterization of the Heme Chaperone CcmE Reveals a Heme Binding Site

CcmE is a heme chaperone that binds heme transiently in the periplasm of Escherichia coli and delivers it to newly synthesized and exported c-type cytochromes. The chemical nature of the covalent bond between heme and H130 is not known. We have purified soluble histidine-tagged CcmE and present its...

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Détails bibliographiques
Auteurs principaux: Enggist, Elisabeth, Schneider, Michael J., Schulz, Henk, Thöny-Meyer, Linda
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2003
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC141962/
https://ncbi.nlm.nih.gov/pubmed/12486054
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.185.1.175-183.2003
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