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Simple method for the preparation of single cell suspensions from normal and tumorous rat colonic mucosa.

Viable single cell suspensions from rat colonic epithelium were obtained by using phosphate buffered saline containing 0-2 M mannitol. The method, which requires no prior enzyme treatment, provides undamaged cells in high yield within one hour. The procedure was also applied to neoplastic rat coloni...

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Bibliografische gegevens
Hoofdauteurs: Perret, V, Lev, R, Pigman, W
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1977
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1411491/
https://ncbi.nlm.nih.gov/pubmed/873323
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