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Baculovirus Alkaline Nuclease Possesses a 5′→3′ Exonuclease Activity and Associates with the DNA-Binding Protein LEF-3

Alkaline nuclease (AN) of the Autographa californica multiple-capsid nucleopolyhedrovirus (AcMNPV) (open reading frame 133) was expressed in recombinant baculovirus as a His(6)-tagged fusion and purified by sequential chromatography on Ni-NTA-agarose, DEAE-Toyopearl, and heparin-Sepharose. At all st...

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Detalles Bibliográficos
Publicado en:J Virol
Principais autores: Mikhailov, Victor S., Okano, Kazuhiro, Rohrmann, George F.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 2003
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC141126/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12551981/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JVI.77.4.2436-2444.2003
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