Baculovirus Alkaline Nuclease Possesses a 5′→3′ Exonuclease Activity and Associates with the DNA-Binding Protein LEF-3
Alkaline nuclease (AN) of the Autographa californica multiple-capsid nucleopolyhedrovirus (AcMNPV) (open reading frame 133) was expressed in recombinant baculovirus as a His(6)-tagged fusion and purified by sequential chromatography on Ni-NTA-agarose, DEAE-Toyopearl, and heparin-Sepharose. At all st...
Gardado en:
| Publicado en: | J Virol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
2003
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC141126/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12551981/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JVI.77.4.2436-2444.2003 |
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