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Visualization of coupled protein folding and binding in bacteria and purification of the heterodimeric complex
During overexpression of recombinant proteins in Escherichia coli, misfolded proteins often aggregate and form inclusion bodies. If an aggregation-prone recombinant protein is fused upstream (as an N-terminal fusion) to GFP, aggregation of the recombinant protein domain also leads to misfolding of t...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The National Academy of Sciences
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC141020/ https://ncbi.nlm.nih.gov/pubmed/12515863 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0236088100 |
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