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Trace fluorescent labeling for high-throughput crystallography

Covalent labeling of macromolecules with trace levels (<1%) of a fluorescent dye is proposed as a means to facilitate finding or detecting crystals in crystallization drops. To test the effects of labeled protein concentration on the resulting X-ray diffraction data, experiments were carried out...

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Bibliografiske detaljer
Main Authors: Forsythe, Elizabeth, Achari, Aniruddha, Pusey, Marc L.
Format: Artigo
Sprog:Inglês
Udgivet: 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1409766/
https://ncbi.nlm.nih.gov/pubmed/16510981
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S0907444906000813
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