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Quantitative Detection of Mycoplasma genitalium from First-Pass Urine of Men with Urethritis and Asymptomatic Men by Real-Time PCR

We developed a TaqMan-based real-time PCR assay for quantifying Mycoplasma genitalium. This assay is able to specifically quantify concentrations of the M. genitalium 16S rRNA gene ranging from 10(7) to 10 copies/reaction. Using the TaqMan assay, we quantified the M. genitalium 16S rRNA gene in firs...

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Bibliografiske detaljer
Main Authors: Yoshida, Takashi, Deguchi, Takashi, Ito, Masayasu, Maeda, Shin-Ichi, Tamaki, Masayoshi, Ishiko, Hiroaki
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2002
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC140368/
https://ncbi.nlm.nih.gov/pubmed/11923372
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.4.1451-1455.2002
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