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Rapid Detection of Shiga Toxin-Producing Bacteria in Feces by Multiplex PCR with Molecular Beacons on the Smart Cycler

We have developed a rapid (1-h) real-time fluorescence-based PCR assay with the Smart Cycler thermal cycler (Cepheid, Sunnyvale, Calif.) for the detection of Shiga toxin-producing Escherichia coli (STEC), as well as other Shiga toxin-producing bacteria. Based on multiple-sequence alignments, we have...

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Udgivet i:J Clin Microbiol
Principais autores: Bélanger, Simon D., Boissinot, Maurice, Ménard, Christian, Picard, François J., Bergeron, Michel G.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2002
Fag:
Bacteriology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC140333/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11923369/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.4.1436-1440.2002
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