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Single-step purification of full-length human androgen receptor
The full-length human androgen receptor with an N-terminal biotin acceptor peptide tag was overexpressed in Spodoptera frugiperda cells in the presence of 1 µM dihydrotestosterone. Site-specific biotinylation of BAP was achieved in vivo by co-expression of E. coli biotin holoenzyme synthetase. The a...
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| Main Authors: | , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
The Nuclear Receptor Signaling Atlas
2005
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1402216/ https://ncbi.nlm.nih.gov/pubmed/16604169 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1621/nrs.03001 |
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