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Protocol for Real-Time PCR Identification of Anthrax Spores from Nasal Swabs after Broth Enrichment

A mass-screening protocol for the diagnosis of anthrax from nasal swabs based on an enrichment step in liquid medium was devised. Incubation for growth was performed in autoclavable vials and racks which allow real-time PCR analysis of sterilized cultures. A dual-color PCR was set up with primers an...

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Bibliografske podrobnosti
izdano v:J Clin Microbiol
Principais autores: Oggioni, Marco R., Meacci, Francesca, Carattoli, Alessandra, Ciervo, Alessandra, Orru, Germano, Cassone, Antonio, Pozzi, Gianni
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 2002
Teme:
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC139649/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12409358/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.11.3956-3963.2002
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