Molecular Cloning and Expression of Mn(2+)-Dependent Sphingomyelinase/Hemolysin of an Aquatic Bacterium, Pseudomonas sp. Strain TK4

We report here the molecular cloning and expression of a hemolytic sphingomyelinase from an aquatic bacterium, Pseudomonas sp. strain TK4. The sphingomyelinase gene was found to consist of 1,548 nucleotides encoding 516 amino acid residues. The recombinant 57.7-kDa enzyme hydrolyzed sphingomyelin bu...

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Detalhes bibliográficos
Main Authors: Sueyoshi, Noriyuki, Kita, Katsuhiro, Okino, Nozomu, Sakaguchi, Keishi, Nakamura, Takashi, Ito, Makoto
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Enzymes and Proteins
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC139580/
https://ncbi.nlm.nih.gov/pubmed/11751833
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.184.2.540-546.2002
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