Depletion of Free 30S Ribosomal Subunits in Escherichia coli by Expression of RNA Containing Shine-Dalgarno-Like Sequences

We have constructed synthetic coding sequences for the expression of poly(α,l-glutamic acid) (PLGA) as fusion proteins with dihydrofolate reductase (DHFR) in Escherichia coli. These PLGA coding sequences use both GAA and GAG codons for glutamic acid and contain sequence elements (5′-GAGGAGG-3′) that...

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Detalhes bibliográficos
Main Authors: Mawn, Mary V., Fournier, Maurille J., Tirrell, David A., Mason, Thomas L.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Transcriptional Regulation
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC139575/
https://ncbi.nlm.nih.gov/pubmed/11751827
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.184.2.494-502.2002
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