Detection and Genotyping of SHV β-Lactamase Variants by Mass Spectrometry after Base-Specific Cleavage of In Vitro-Generated RNA Transcripts

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) after base-specific cleavage of PCR-amplified and in vitro-transcribed bla(SHV) genes was used for the identification and genotyping of SHV β-lactamases. For evaluation, bla(SHV) stretches of 21 clinical Ente...

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Détails bibliographiques
Auteurs principaux: Stürenburg, Enno, Storm, Niels, Sobottka, Ingo, Horstkotte, Matthias A., Scherpe, Stefanie, Aepfelbacher, Martin, Müller, Susanne
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2006
Sujets:
Bacteriology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1393099/
https://ncbi.nlm.nih.gov/pubmed/16517875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.44.3.909-915.2006
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