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Construction and Complementation of In-Frame Deletions of the Essential Escherichia coli Thymidylate Kinase Gene
This work reports the construction of Escherichia coli in-frame deletion strains of tmk, which encodes thymidylate kinase, Tmk. The tmk gene is located at the third position of a putative five-gene operon at 24.9 min on the E. coli chromosome, which comprises the genes pabC, yceG, tmk, holB, and ycf...
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
American Society for Microbiology
2006
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1392977/ https://ncbi.nlm.nih.gov/pubmed/16461678 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.2.1288-1294.2006 |
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