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Unreliable Measurement of Basophil Maximum Leukotriene Release with the Bühlmann CAST 2000 Enzyme-Linked Immunosorbent Assay Kit

The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive resu...

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Autori principali: Burastero, S. E., Paolucci, C., Breda, D., Monasterolo, G., Rossi, R. E., Vangelista, L.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2006
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1391964/
https://ncbi.nlm.nih.gov/pubmed/16522787
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CVI.13.3.420-422.2006
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