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False-Positive Results in a Recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) Nucleocapsid-Based Western Blot Assay Were Rectified by the Use of Two Subunits (S1 and S2) of Spike for Detection of Antibody to SARS-CoV

To evaluate the reactivity of the recombinant proteins expressed in Escherichia coli strain BL21(DE3), a Western blot assay was performed by using a panel of 78 serum samples obtained, respectively, from convalescent-phase patients infected with severe acute respiratory syndrome-associated coronavir...

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Detalhes bibliográficos
Main Authors: Maache, Mimoun, Komurian-Pradel, Florence, Rajoharison, Alain, Perret, Magali, Berland, Jean-Luc, Pouzol, Stéphane, Bagnaud, Audrey, Duverger, Blandine, Xu, Jianguo, Osuna, Antonio, Paranhos-Baccalà, Glaucia
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1391961/
https://ncbi.nlm.nih.gov/pubmed/16522785
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CVI.13.3.409-414.2006
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