Overlapping PCR for Bidirectional PCR Amplification of Specific Alleles: A Rapid One-Tube Method for Simultaneously Differentiating Homozygotes and Heterozygotes

Rapid detection of single-base changes is fundamental to molecular medicine. PASA (PCR amplification of specific alleles) is a rapid method of genotyping single-base changes, but one reaction is required for each allele. Bidirectional PASA (Bi-PASA) was developed to distinguish between homozygotes a...

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Detalles Bibliográficos

Publicado en:	Genome Res
Principais autores:	Liu, Qiang, Thorland, Erik C., Heit, John A., Sommer, Steve S.
Formato:	Artigo
Idioma:	Inglês
Publicado:	Cold Spring Harbor Laboratory Press 1997
Assuntos:	Genome Methods
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC139149/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9110178/ https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gr.7.4.389
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