Overlapping PCR for Bidirectional PCR Amplification of Specific Alleles: A Rapid One-Tube Method for Simultaneously Differentiating Homozygotes and Heterozygotes

Rapid detection of single-base changes is fundamental to molecular medicine. PASA (PCR amplification of specific alleles) is a rapid method of genotyping single-base changes, but one reaction is required for each allele. Bidirectional PASA (Bi-PASA) was developed to distinguish between homozygotes a...

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Bibliografiska uppgifter

I publikationen:	Genome Res
Huvudupphov:	Liu, Qiang, Thorland, Erik C., Heit, John A., Sommer, Steve S.
Materialtyp:	Artigo
Språk:	Inglês
Utgiven:	Cold Spring Harbor Laboratory Press 1997
Ämnen:	Genome Methods
Länkar:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC139149/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9110178/ https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gr.7.4.389
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