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Single-Nucleotide Polymorphism Identification Assays Using a Thermostable DNA Polymerase and Delayed Extraction MALDI-TOF Mass Spectrometry

We report a simple method, the PinPoint assay, for detecting and identifying single-base variations (polymorphisms) at specific locations within DNA sequences. An oligonucleotide primer is annealed to the target DNA immediately upstream of the polymorphic site and is extended by a single base in the...

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Detalles Bibliográficos
Autores principales: Haff, Lawrence A., Smirnov, Igor P.
Formato: Artigo
Lenguaje:Inglês
Publicado: Cold Spring Harbor Laboratory Press 1997
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC139147/
https://ncbi.nlm.nih.gov/pubmed/9110177
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