Comparison of PCR and Plaque Assay for Detection and Enumeration of Coliphage in Polluted Marine Waters

A total of 68 marine samples from various sites impacted by sewage and storm waters were analyzed by both the plaque assay and a reverse transcriptase (RT) PCR technique for F(sup+)-specific coliphage. The coliphage levels detected by the plaque assay averaged 1.90 x 10(sup4) PFU/100.0 ml. Using a m...

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Main Authors: Rose, J. B., Zhou, X., Griffin, D. W., Paul, J. H.
Format: Artigo
Sprog:Inglês
Udgivet: 1997
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1389293/
https://ncbi.nlm.nih.gov/pubmed/16535737
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