A Method for Purification of Clostridium perfringens Phospholipase C from Recombinant Bacillus subtilis Cells

We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture.

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Detalles Bibliográficos
Autores principales: Hirata, Y., Minami, J., Koyama, M., Matsushita, O., Katayama, S., Jin, F., Maeta, H., Okabe, A.
Formato: Artigo
Lenguaje:Inglês
Publicado: 1995
Materias:
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1388606/
https://ncbi.nlm.nih.gov/pubmed/16535170
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