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Combining computational and experimental screening for rapid optimization of protein properties

We present a combined computational and experimental method for the rapid optimization of proteins. Using β-lactamase as a test case, we redesigned the active site region using our Protein Design Automation technology as a computational screen to search the entire sequence space. By eliminating sequ...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Hayes, Robert J., Bentzien, Jörg, Ary, Marie L., Hwang, Marian Y., Jacinto, Jonathan M., Vielmetter, Jost, Kundu, Anirban, Dahiyat, Bassil I.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: National Academy of Sciences 2002
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC138541/
https://ncbi.nlm.nih.gov/pubmed/12446841
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.212627499
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