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Combining computational and experimental screening for rapid optimization of protein properties
We present a combined computational and experimental method for the rapid optimization of proteins. Using β-lactamase as a test case, we redesigned the active site region using our Protein Design Automation technology as a computational screen to search the entire sequence space. By eliminating sequ...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC138541/ https://ncbi.nlm.nih.gov/pubmed/12446841 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.212627499 |
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