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Hybridization of 2′-O-methyl and 2′-deoxy molecular beacons to RNA and DNA targets

Molecular beacons are stem–loop hairpin oligonucleotide probes labeled with a fluorescent dye at one end and a fluorescence quencher at the other end; they can differentiate between bound and unbound probes in homogeneous hybridization assays with a high signal-to-background ratio and enhanced speci...

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Detalles Bibliográficos
Main Authors: Tsourkas, Andrew, Behlke, Mark A., Bao, Gang
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2002
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC137950/
https://ncbi.nlm.nih.gov/pubmed/12466541
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