Proliferating cell nuclear antigen in oesophageal diseases; correlation with transforming growth factor alpha expression.

This study was designed to correlate mucosal proliferation in Barrett's oesophagus with expression of a growth promoting peptide, transforming growth factor alpha (TGF alpha). Oesophageal mucosa was studied from 50 patients with oesophageal disease who had been treated by oesophagectomy. Histol...

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Hlavní autoři: Jankowski, J, McMenemin, R, Yu, C, Hopwood, D, Wormsley, K G
Médium: Artigo
Jazyk:Inglês
Vydáno: 1992
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1379282/
https://ncbi.nlm.nih.gov/pubmed/1351861
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spelling pubmed-13792822007-06-11 Proliferating cell nuclear antigen in oesophageal diseases; correlation with transforming growth factor alpha expression. Jankowski, J McMenemin, R Yu, C Hopwood, D Wormsley, K G Gut Research Article This study was designed to correlate mucosal proliferation in Barrett's oesophagus with expression of a growth promoting peptide, transforming growth factor alpha (TGF alpha). Oesophageal mucosa was studied from 50 patients with oesophageal disease who had been treated by oesophagectomy. Histological analysis showed a range of oesophageal pathology - 18 patients had gastric type Barrett's mucosa, 18 had intestinal type Barrett's mucosa, and 14 had oesophageal adenocarcinomas. Sections were stained immunohistochemically for proliferating cell nuclear antigen (PCNA) (an index of cellular proliferation) and TGF alpha. PCNA immunostaining was seen mainly in the basal cells of the neck/foveolar epithelial compartment of the glands in Barrett's oesophagus. However, in mucosa with high grade dysplasia, the proliferative compartment extended upwards into the superficial layers of the glands. At least 2000 cells were counted in each patient to determine the proportion with PCNA immunoreactivity (PCNA labelling index). The labelling index was highest in adenocarcinoma (25%) and in Barrett's intestinal type mucosa with high grade dysplasia (26%) compared with intestinal type mucosa with no significant dysplasia (20%) and Barrett's gastric type mucosa (12%). There was a significant positive correlation between PCNA labelling indices and TGF alpha expression in Barrett's mucosa (p less than 0.01). In glands showing high grade dysplasia, TGF alpha immunoreactivity was seen in the same regions of the glands as PCNA immunoreactivity, indicating the possibility of involvement of TGF alpha in (pre) neoplastic proliferation in Barrett's oesophagus. 1992-05 /pmc/articles/PMC1379282/ /pubmed/1351861 Text en
institution US NLM
collection PubMed Central
language Inglês
format Artigo
topic Research Article
spellingShingle Research Article
Jankowski, J
McMenemin, R
Yu, C
Hopwood, D
Wormsley, K G
Proliferating cell nuclear antigen in oesophageal diseases; correlation with transforming growth factor alpha expression.
description This study was designed to correlate mucosal proliferation in Barrett's oesophagus with expression of a growth promoting peptide, transforming growth factor alpha (TGF alpha). Oesophageal mucosa was studied from 50 patients with oesophageal disease who had been treated by oesophagectomy. Histological analysis showed a range of oesophageal pathology - 18 patients had gastric type Barrett's mucosa, 18 had intestinal type Barrett's mucosa, and 14 had oesophageal adenocarcinomas. Sections were stained immunohistochemically for proliferating cell nuclear antigen (PCNA) (an index of cellular proliferation) and TGF alpha. PCNA immunostaining was seen mainly in the basal cells of the neck/foveolar epithelial compartment of the glands in Barrett's oesophagus. However, in mucosa with high grade dysplasia, the proliferative compartment extended upwards into the superficial layers of the glands. At least 2000 cells were counted in each patient to determine the proportion with PCNA immunoreactivity (PCNA labelling index). The labelling index was highest in adenocarcinoma (25%) and in Barrett's intestinal type mucosa with high grade dysplasia (26%) compared with intestinal type mucosa with no significant dysplasia (20%) and Barrett's gastric type mucosa (12%). There was a significant positive correlation between PCNA labelling indices and TGF alpha expression in Barrett's mucosa (p less than 0.01). In glands showing high grade dysplasia, TGF alpha immunoreactivity was seen in the same regions of the glands as PCNA immunoreactivity, indicating the possibility of involvement of TGF alpha in (pre) neoplastic proliferation in Barrett's oesophagus.
author Jankowski, J
McMenemin, R
Yu, C
Hopwood, D
Wormsley, K G
author_facet Jankowski, J
McMenemin, R
Yu, C
Hopwood, D
Wormsley, K G
author_sort Jankowski, J
title Proliferating cell nuclear antigen in oesophageal diseases; correlation with transforming growth factor alpha expression.
title_short Proliferating cell nuclear antigen in oesophageal diseases; correlation with transforming growth factor alpha expression.
title_full Proliferating cell nuclear antigen in oesophageal diseases; correlation with transforming growth factor alpha expression.
title_fullStr Proliferating cell nuclear antigen in oesophageal diseases; correlation with transforming growth factor alpha expression.
title_full_unstemmed Proliferating cell nuclear antigen in oesophageal diseases; correlation with transforming growth factor alpha expression.
title_sort proliferating cell nuclear antigen in oesophageal diseases; correlation with transforming growth factor alpha expression.
publishDate 1992
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1379282/
https://ncbi.nlm.nih.gov/pubmed/1351861
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