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An allelic series of mutations in Smad2 and Smad4 identified in a genotype-based screen of N-ethyl-N- nitrosourea-mutagenized mouse embryonic stem cells

Using selectable genes as proof of principle, a new high-throughput genotype-based mutation screen in mouse embryonic stem (ES) cells was developed [Chen et al. (2002) Nat. Genet. 24, 314–317]. If expanded to nonselectable genes, this approach would allow one to proceed quickly from sequence to whol...

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Главные авторы: Vivian, Jay L., Chen, Yijing, Yee, Della, Schneider, Elizabeth, Magnuson, Terry
Формат: Artigo
Язык:Inglês
Опубликовано: National Academy of Sciences 2002
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC137753/
https://ncbi.nlm.nih.gov/pubmed/12432092
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.242474199
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