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Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection

Locked nucleic acids (LNAs) are synthetic nucleic acid analogs that bind to complementary target molecules (DNA, RNA or LNA) with very high affinity. At the same time, this binding affinity is decreased substantially when the hybrids thus formed contain even a single mismatched base pair. We have ex...

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Detalhes bibliográficos
Main Authors: Simeonov, Anton, Nikiforov, Theo T.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2002
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC137436/
https://ncbi.nlm.nih.gov/pubmed/12202779
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