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A double reporter assay for detecting changes in the ratio of spliced and unspliced mRNA in mammalian cells

Current methods for measuring the efficiency of splicing in mammalian cells rely on either direct analysis of the RNA, which does not lend itself to rapid assays, or on single reporter functions that are subject to numerous intrinsic variables. If two protein activities are encoded within a single r...

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Autori principali: Nasim, Md. Talat, Chowdhury, H. M., Eperon, I. C.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2002
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC137153/
https://ncbi.nlm.nih.gov/pubmed/12384611
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