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A double reporter assay for detecting changes in the ratio of spliced and unspliced mRNA in mammalian cells

Current methods for measuring the efficiency of splicing in mammalian cells rely on either direct analysis of the RNA, which does not lend itself to rapid assays, or on single reporter functions that are subject to numerous intrinsic variables. If two protein activities are encoded within a single r...

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Detalhes bibliográficos
Main Authors: Nasim, Md. Talat, Chowdhury, H. M., Eperon, I. C.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC137153/
https://ncbi.nlm.nih.gov/pubmed/12384611
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