Complete MALDI-ToF MS analysis of cross-linked peptide–RNA oligonucleotides derived from nonlabeled UV-irradiated ribonucleoprotein particles

Protein–RNA cross-linking combined with mass spectrometry is a powerful tool to elucidate hitherto noncharacterized protein–RNA contacts in ribonucleoprotein particles, as, for example, within spliceosomes. Here, we describe an improved methodology for the sequence analysis of purified peptide–RNA o...

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Autors principals: KÜHN-HÖLSKEN, EVA, LENZ, CHRISTOF, SANDER, BJÖRN, LÜHRMANN, REINHARD, URLAUB, HENNING
Format: Artigo
Idioma:Inglês
Publicat: Copyright 2005 by RNA Society 2005
Matèries:
Method
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370879/
https://ncbi.nlm.nih.gov/pubmed/16314460
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2176605
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