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RNA–protein interactions in the yeast three-hybrid system: Affinity, sensitivity, and enhanced library screening
The yeast three-hybrid system has become a useful tool in analyzing RNA–protein interactions. An RNA sequence is tested in combination with an RNA-binding protein linked to a transcription activation domain (AD). A productive RNA–protein interaction activates a reporter gene in vivo. The system has...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Copyright 2005 by RNA Society
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1370711/ https://ncbi.nlm.nih.gov/pubmed/15613539 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.7202705 |
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