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Crosslinking of 4.5S RNA to the Escherichia coli ribosome in the presence or absence of the protein Ffh.
Radioactively labeled 4.5S RNA containing statistically distributed 4-thiouridine residues in place of normal uridine was prepared by T7 transcription. The ability of this modified 4.5S RNA to form a complex with the protein Ffh was demonstrated by a gel shift assay. The modified 4.5S RNA, with or w...
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| Main Authors: | , , , , , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2002
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1370282/ https://ncbi.nlm.nih.gov/pubmed/12022228 |
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