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Chemical and enzymatic synthesis of tRNAs for high-throughput crystallization.

Preparation of large quantities of RNA molecules of a defined sequence is a prerequisite for biophysical analysis, and is particularly important to the determination of high-resolution structure by X-ray crystallography. We describe improved methods for the production of multimilligram quantities of...

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Detalhes bibliográficos
Main Authors: Sherlin, L D, Bullock, T L, Nissan, T A, Perona, J J, Lariviere, F J, Uhlenbeck, O C, Scaringe, S A
Formato: Artigo
Idioma:Inglês
Publicado em: 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370207/
https://ncbi.nlm.nih.gov/pubmed/11720294
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