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Estimation of SELEX pool size by measurement of DNA renaturation rates.

We report a general method of measuring the complexity of SELEX pools. In analogy to measurements of genome size by C0t analysis, the complexity of a SELEX pool is measured by determining the reannealing rate of its double-stranded PCR product. We applied this technique to study the selection dynami...

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Autori principali: Charlton, J, Smith, D
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1999
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1369854/
https://ncbi.nlm.nih.gov/pubmed/10573123
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