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A novel 5 displacement spin-labeling technique for electron paramagnetic resonance spectroscopy of RNA.

An RNA spin-labeling technique was developed using the well-characterized interaction between the HIV Rev peptide and the Rev response element (RRE) RNA as a model system. Spin-labeled RNA molecules were prepared by incorporating guanosine monophosphorothioate (GMPS) at the 5' end using T7 RNA...

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Detalhes bibliográficos
Main Authors: Macosko, J C, Pio, M S, Tinoco, I, Shin, Y K
Formato: Artigo
Idioma:Inglês
Publicado em: 1999
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1369839/
https://ncbi.nlm.nih.gov/pubmed/10496217
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