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Construction of an in vivo-regulated U6 snRNA transcription unit as a tool to study U6 function.

U6 snRNA is the only spliceosomal snRNA transcribed by RNA polymerase III in yeast. We have constructed a regulated U6 snRNA transcription unit by introducing the binding site for the Escherichia coli lacI repressor protein in the U6 snRNA promoter. GAL-induced expression of lacI protein led to a de...

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Detalhes bibliográficos
Main Authors: Luukkonen, B G, Séraphin, B
Formato: Artigo
Idioma:Inglês
Publicado em: 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1369612/
https://ncbi.nlm.nih.gov/pubmed/9570323
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