Paired Charge-to-Alanine Mutagenesis of Dengue Virus Type 4 NS5 Generates Mutants with Temperature-Sensitive, Host Range, and Mouse Attenuation Phenotypes

Charge-to-alanine mutagenesis of dengue virus type 4 (DEN4) NS5 gene generated a collection of attenuating mutations for potential use in a recombinant live attenuated DEN vaccine. Codons for 80 contiguous pairs of charged amino acids in NS5 were individually mutagenized to create uncharged pairs of...

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Bibliografiske detaljer

Udgivet i:	J Virol
Principais autores:	Hanley, Kathryn A., Lee, Jay J., Blaney, Joseph E., Murphy, Brian R., Whitehead, Stephen S.
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 2002
Fag:	Vaccines and Antiviral Agents
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC136841/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11752143/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JVI.76.2.525-531.2002
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