Paired Charge-to-Alanine Mutagenesis of Dengue Virus Type 4 NS5 Generates Mutants with Temperature-Sensitive, Host Range, and Mouse Attenuation Phenotypes

Charge-to-alanine mutagenesis of dengue virus type 4 (DEN4) NS5 gene generated a collection of attenuating mutations for potential use in a recombinant live attenuated DEN vaccine. Codons for 80 contiguous pairs of charged amino acids in NS5 were individually mutagenized to create uncharged pairs of...

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Bibliographic Details
Main Authors: Hanley, Kathryn A., Lee, Jay J., Blaney, Joseph E., Murphy, Brian R., Whitehead, Stephen S.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2002
Subjects:
Vaccines and Antiviral Agents
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC136841/
https://ncbi.nlm.nih.gov/pubmed/11752143
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.76.2.525-531.2002
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