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In the Bacillus stearothermophilus DnaB-DnaG Complex, the Activities of the Two Proteins Are Modulated by Distinct but Overlapping Networks of Residues

We demonstrate the primase activity of Bacillus stearothermophilus DnaG and show that it initiates at 3′-ATC-5′ and 3′-ATT-5′ sites synthesizing primers that are 22 or 23 nucleotides long. In the presence of the helicase DnaB the size distribution of primers is different, and a range of additional s...

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Bibliographic Details
Main Authors: Thirlway, Jenny, Soultanas, Panos
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2006
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1367256/
https://ncbi.nlm.nih.gov/pubmed/16452437
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.188.4.1534-1539.2006
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