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Measuring Single-Bond Rupture Forces Using High Electric Fields in Microfluidic Channels and DNA Oligomers as Force Tags

The disruption force of specific biotin-streptavidin bonds was determined using DNA oligomers as force tags. Forces were generated by an electric field acting on a biotinylated fluorescently labeled DNA oligomer. DNA oligomers were immobilized via biotin-streptavidin bonds on the walls of microfluid...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Breisch, Stefanie, Gonska, Julian, Deissler, Helmut, Stelzle, Martin
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Biophysical Society 2005
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC1366719/
https://ncbi.nlm.nih.gov/pubmed/16040764
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.066795
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