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Mechanical Studies of Single Ribosome/mRNA Complexes

Methodology was developed for specifically anchoring Escherichia coli 70S ribosomes onto a chemically modified, cysteine-reactive glass surface. Immobilized ribosomes maintain the capability of binding a polyuridylic acid (poly(U)) template, enabling investigation of mechanical properties of individ...

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Detalhes bibliográficos
Main Authors: Vanzi, Francesco, Takagi, Yasuharu, Shuman, Henry, Cooperman, Barry S., Goldman, Yale E.
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1366694/
https://ncbi.nlm.nih.gov/pubmed/15951374
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.056283
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