RNA-Binding Activity of the Rotavirus Phosphoprotein NSP5 Includes Affinity for Double-Stranded RNA

Phosphoprotein NSP5 is a component of replication intermediates that catalyze the synthesis of the segmented double-stranded RNA (dsRNA) rotavirus genome. To study the role of the protein in viral replication, His-tagged NSP5 was expressed in bacteria and purified by affinity chromatography. In vitr...

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Detalhes bibliográficos

Publicado no:	J Virol
Principais autores:	Vende, Patrice, Taraporewala, Zenobia F., Patton, John T.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2002
Assuntos:	Replication
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC136158/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11967345/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JVI.76.10.5291-5299.2002
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