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Isolation of the Gene for the B(12)-Dependent Ribonucleotide Reductase from Anabaena sp. Strain PCC 7120 and Expression in Escherichia coli

The gene for ribonucleotide reductase from Anabaena sp. strain PCC 7120 was identified and expressed in Escherichia coli. This gene codes for a 1,172-amino-acid protein that contains a 407-amino-acid intein. The intein splices itself from the protein when it is expressed in E. coli, yielding an acti...

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Detalhes bibliográficos
Main Authors: Gleason, Florence K., Olszewski, Neil E.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC135420/
https://ncbi.nlm.nih.gov/pubmed/12426342
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.184.23.6544-6550.2002
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