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In Vitro Alginate Polymerization and the Functional Role of Alg8 in Alginate Production by Pseudomonas aeruginosa
An enzymatic in vitro alginate polymerization assay was developed by using (14)C-labeled GDP-mannuronic acid as a substrate and subcellular fractions of alginate overproducing Pseudomonas aeruginosa FRD1 as a polymerase source. The highest specific alginate polymerase activity was detected in the en...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2006
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1352289/ https://ncbi.nlm.nih.gov/pubmed/16391057 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.1.298-305.2006 |
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