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Development and Validation of a Real-Time PCR Method To Quantify Rumen Protozoa and Examination of Variability between Entodinium Populations in Sheep Offered a Hay-Based Diet

PCR and real-time PCR primers for the 18S rRNA gene of rumen protozoa (Entodinium and Dasytricha spp.) were designed, and their specificities were tested against a range of rumen microbes and protozoal groups. External standards were prepared from DNA extracts of a rumen matrix containing known numb...

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Detalhes bibliográficos
Main Authors: Skillman, Lucy C., Toovey, Andrew F., Williams, Andrew J., Wright, André-Denis G.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1352179/
https://ncbi.nlm.nih.gov/pubmed/16391043
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.1.200-206.2006
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