Development and Evaluation of a Real-Time PCR Assay Targeting the Type III Secretion System of Burkholderia pseudomallei

Here we report on the development of a discriminatory real-time assay for the rapid identification of Burkholderia pseudomallei isolates and the evaluation of this assay for sensitivity against related species and detection in spiked human blood samples. The assay targets a 115-base-pair region with...

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Bibliographic Details
Main Authors: Novak, Ryan T., Glass, Mindy B., Gee, Jay E., Gal, Daniel, Mayo, Mark J., Currie, Bart J., Wilkins, Patricia P.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2006
Subjects:
Bacteriology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1351940/
https://ncbi.nlm.nih.gov/pubmed/16390953
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.44.1.85-90.2006
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