Development and Evaluation of a Real-Time PCR Assay Targeting the Type III Secretion System of Burkholderia pseudomallei

Here we report on the development of a discriminatory real-time assay for the rapid identification of Burkholderia pseudomallei isolates and the evaluation of this assay for sensitivity against related species and detection in spiked human blood samples. The assay targets a 115-base-pair region with...

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Detalhes bibliográficos
Main Authors: Novak, Ryan T., Glass, Mindy B., Gee, Jay E., Gal, Daniel, Mayo, Mark J., Currie, Bart J., Wilkins, Patricia P.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Bacteriology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1351940/
https://ncbi.nlm.nih.gov/pubmed/16390953
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.44.1.85-90.2006
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